Determination of sugar content of some inbread sweet corn lines and screening with ssr markers

In this study we evaluated 26 sweet corn inbred lines which is obtained from the U.S. Department of Agriculture and 23 sweet corn inbred lines developed by Ege University, Faculty of Agriculture, Field Crops Department. In total 49 different sweet corn inbred lines were planted with lattice experimetal design in 2015. The leaf samples were taken when they were within 4-5 leaf stage for DNA isolation. After harvesting of the corn cobs, total sugar content in the kernel were measured by Luff-Schoorl method. At the same time, moleculer screenings were carried out by using 9 SSR marker which is related to sugar content genes in 49 inbred sweet corn lines. The total sugar content were ranged from 0,66 % to 16,84 % in 49 sweet corn inbred lines. For genes which are related to the sugar content of sweet corn was determined to more suitable umc1031 primer for su gene, phi44 primer for sh-1 gene, umc2276 primer for sh-2 gene and phi328175 primer for ea-1 gene. In addition to, since dent corn sugar content is the most 4.0 %, the total sugar content of 24 inbred lines in this study were found higher than 4.0 %. We concluded that these genotypes can be used as parental genotypes for improved of hybrid sweet corn cultivars.