Investigation of the potential of some plant extracts in inhibition of some important enzymes in metabolic diseases

Natural products have potential to produce compounds, which can be used against various diseases. While taking into account widespread drug resistance it is important to investigate new antimicrobial substances. In this study, the antimicrobial activities of the extracts of three macrofungi, namely Fomes fomentarius (L.) Fr., Phellinus hartigii (Allesch. & Schnabl) Pat. and Fomitopsis pinicola (Sw.) P. Karst., which were extracted by using six different solvents (water, methanol, chloroform, acetone, petroleum ether and n-hexane), were examined against twelve different Escherichia coli strains, where one of these strains was a standard strain (E. coli ATCC 25922) and eleven strain were clinical isolates presenting different resistance profiles. For determining the antimicrobial activity of the extracts minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) tests were used. In addition, time-kill kinetics test was conducted for selected extracts. As a result it was observed that extracts have both bacteriostatic or bacteriocidal activity against E. coli strains. Further researched should be conducted to determine the chemical composition of the extracts and the mode of action.