Synthesis of some novel purine derivatives, evaluation of their structures by spectroscopic methods and investigation of their biological activity

In this study, some novel biologically active purine derivatives having different substituents at position 9 were synthesized. The substitution reaction of 6-chloropurine with potassium thiocyanate gave 6-thiocyanato-9H-purine. Treatment of 6-thiocyanato-9H-purine with appropriate alkyl halides in the presence of potassium carbonate generated 9-alkyl-6-thiocyanatopurines. 9-alkyl-N-(1H-tetrazol-5-yl)-9H-purin-6-amines have been synthesized from the reaction of 6-chloro-9-alkyl-9H-purines with 5-aminotetrazole, and 9-alkyl-6-(1-methyl-1H-tetrazol-5-ylthio)-9H-purines have been synthesized from the reaction of 6-chloro-9-alkyl-9H-purines with 1-methyl-5-mercaptotetrazole. The structures of the synthesized compounds were confirmed by spectroscopic techniques (FT-IR, 1H-NMR, 13C-APT, HR-MS). The antimicrobial activity of the synthesized compounds and the effect of the compounds on DNA were investigated.Key Words : Purine derivative, antimetabolite, tetrazole, DNA interaction, antimicrobial activity