Beyin rejenerasyonu ve glioma moleküler mekanizmalarının zebrabalığı modeli kullanılarak gliomalarda terapötik yaklaşımlar geliştirilmesi için karşılaştırmalı olarak analizi

Studies have shown that events such as proliferation, renewal of cells and differentiation are shared between cancer and regeneration. However, while these events are terminated properly in regeneration, they cannot be halted in cancer and the cells undergo proliferation consistently resulting with abnormal tissue integrity and abnormal tissue mass. Unlike mammals, zebrafish has the ability to regenerate many organs and tissues, including brain tissue. This feature has made zebrafish a suitable model organism for vertebral regeneration studies. This study aims to investigate the mechanisms involved in regeneration in early wound healing, early proliferation and differentiation stages in telencephalon tissue at the whole transcriptome level and to elucidate the relationship between cancer and regeneration in brain tissue by comparing these results with human brain cancers obtained from cancer databases. In this study, various molecular biology techniques such as micro-manipulation techniques, histological techniques, qPCR and RNA-sequencing were used. Bioinformatics analyses, including quality control, mapping to the reference genome, read counting, detection of differentially expressed genes, and comparison of results with cancer data from cancer databases were performed to analyse RNA-sequencing data. As a result, (i) the transcriptome of three phases of brain regeneration shows different characteristics; (ii) early wound healing stage is similar to the brain cancers in terms of protein translation, cell development, neurogenesis and synaptic signaling pathways; (iii) early cell proliferation stage and brain cancers are similar each other in terms of cell division and DNA replication; (iv) the differentiation stage and brain cancers resembles each other in terms of nervous system development, vascular development and organ morphogenesis. This study is a resource that investigates three different stages of brain regeneration and two different brain cancers at the whole transcriptome level for the first time. The results will be able to contribute to the literature on the development of new approaches in the treatment of brain cancers, neurodegenerative diseases and brain injury.