Biochemical effects of clothianidin in heart and spleen tissues of Oncorhynchus mykiss

This study, Oncorhynchus mykiss of clothianidine was investigated by considering the potential of inducing neurotoxicity and oxidative stress in the heart and spleen tissues. For this purpose, juvenile O. mykiss 7, 14 and 21 days were determined under the influence of 3, 15, and 30 μg / L concentrations of pesticide, AChE, SOD, CAT, GPx activities, protein amount, MDA, GSH and protein carbonyl levels were determined. It was determined that there was a significant increase in antioxidant enzyme activities, GSH, MDA and protein carbonyl levels. The application of clothianidin reduced the amount of protein while inhibiting tissue AChE activity. After the application, tissue specific responses were observed in somatic indices, and an increase in heart and a decrease in spleen were observed which was more pronounced on extended period of exposure at high concentration. As a result, it is evaluated that alterations occur as an adaptive response in biomarkers, which were analyzed as a result of the application of clothianidin in O. mykiss individuals with induction of neurotoxicity and oxidative stress. The apparent change in the amount of protein carbonyl shows that this parameter can be used as a sensitive indicator, although it is not specific for the chemical type.