Synthesis and characterization of polymeric nanoparticles including bioactive peptide and investigation of nanocosmetic product potential

In this thesis, p(HEMA-MAGA) nanoparticles were synthesized bysurfactant free emulsion polymerization method for controlled release of acetyl octapeptide-3, which mimics the effect mechanism of botulinum neurotoxin and for assessing the potential of nanocosmetic properties. The SEM and AFM were used to determine the morphological characteristics of the synthesizedp(HEMA-MAGA) nanoparticles, and the FT-IR method was used to characterize chemical structures, meanwhile the dimensional analysis was carried out by using a Zeta-Sizer device. According to the SEM and AFM analysis, it was profound that the synthesized polymeric nanoparticles have a homogeneous distribution towards their sizes and a spherical structure. Considering to the zeta-size analysis, it was revealed that the nanoparticle average size was 340,5 nm, and the calculated specific surface area was 350 m2 / g. Time, pH, ionic strength, temperature and concentration parameters were performed to optimize adsorption conditions of acetyl octapeptide-3 loaded nanoparticles. The maximal adsorption amount was 220,69 mg / g at 60 minutes with pH 4.2, 0.1 M ionic strength (NaCl), 1.5 mg / mL concentration and at 25 ° C.ThepH and temperature were carried out to follow up the release conditions of loaded nanoparticles with octapeptide-3. In addition, cytotoxicity tests were done for the toxicity of loaded nanomaterials by using Alamar Blue method. Therefore, based on outcomes of the developed nanocosmetic product, it is prevailed that there is not indication of harmfulness, and it is thought to be used as the alternative of the botulinum neurotoxin. Keywords: Nanocosmetics, Acetyl Octapeptide-3, Botulinum Neurotoxin, Bioactive Peptide, Polymeric Nanoparticles, Controlled Release Systems