Detection and molecular characterization of microorganisms that are able to create synergistic effect on biodegradation and enhance the pesticide activity

Nowadays, with the growing industrial sector and increasing population, a wide variety of chemicals have accumulated in nature. Among the compounds released into nature, pesticides are widely used both in modern agriculture and in food processing, storage, transportation, and marketing. Microorganisms, which are highly preferred in bioremediation technology, have been genetically evolved to activate related reactions to decompose or detoxify various environmental pollutants, including pesticides. In this study, we focused on bacterial bioremediation; the strain named GBS19, which was found to metabolize pesticides that were determined to be a strain of S. marcescens by 16 S rRNA analysis. The whole-genome sequence analysis of S. marcescens GBS19 has been associated with many degradation pathways in the KEGG database. Studies on 25 pesticide active ingredients by LC-MS chromatographic analysis; it has been determined that Serratia marcescens GBS19 strain biologically decomposes some active substances up to 67 %, and it acts on some active substances by providing a synergistic effect, increasing the concentration up to 29 %. Afterward, protein modeling was performed over the allophanate hydrolase gene region encoded by the AtzF gene in the genome of S. marcescens GBS19 to confirm the herbicide degradation of the bacterium. The ligand (atrazine) taken from this modeling was examined in terms of protein-ligand interaction. It was determined with the coordinates that Atrazine can bind to the most Hydrolase A subunit on the hydrolase enzyme comprising 7 separate subunits by "blind placement". Binding affinity values were determined by docking programs according to ligand rotation probabilities, where it could bind on the kcal/mol scaled protein. Then, the results obtained were confirmed by comparing the results of the open-access server used for this purpose on an international scale once again. We believe that our current studies carry important scientific importance at the molecular level of interaction related to bacterial bioremediation processes and its mechanisms at the gene and protein level. Keywords: Serratia marcescens, Serratia marcescens GBS19, Microbial bioremediation, Molecular docking, LC-MS, KEGG database