Böbrek kanserinde WAVE11 gen ekspresyon düzeyinin analizi

Cancer is characterized by uncontrolled cell proliferation and is clearly the most destructive groups of human pathology. Renal cancer was previously thought to exist as a single illness, but it is currently recognized as several distinct cancer types that develop from kidneys, any one of them vary from histological features, disease progression, treatment responses, and genetic basis factors. Several of risk factors influenced renal cancer, involving lifestyle factors, medical condition factors, environmental factors, as well as genetic factors. The WASP family member 1 (WASF1) gene codes for WASP verprolin-homologous protein1 (WAVE1) which is an essential actin cytoskeleton control protein that is highly abundant in the brain. Particularly, WAVE1 is one of the subunits of the WAVE regulatory complex (WRC) that permits activation of the complex of the actin-related protein 2/3 (ARP 2/3) and results in inducing the polymerization of actin that is important in diverse cell events like cellular migration and cellular adhesion. In this paper, we looked at the WAVE1 (also known as WASF1) expression levels within renal cancer. For our work, 11 tissue samples of renal cancer as well as 11 healthy renal tissue samples from the same patient were evaluated. The renal cancer tissues were composed of 6 samples at stage I, 4 samples at stage III, and 1 sample at stage II. To analyze the WAVE1 gene expression level in renal cancer and healthy tissues of any samples, we employed the quantitative real-time polymerase chain reaction (qRT-PCR) method. The findings of this study exhibited that the level of WAVE1 gene was overexpressed in renal cancer tissue samples compared with healthy tissues (Fold change=5.08), which was statistically significant (p = 0.030). As a result, with all of these data, our research findings recommend that the WAVE1 gene does have a significant role in renal cancer progress and growth. Key Words: Actin Cytoskeleton, Kidney Cancer, qRT-PCR, WAVE1.