Identification of bacterial disease agents on commercial seeds of same plants belongs to Brassicaceae family

Sixteen different seed lot samples from Brassicaceous plants (13 cabbage [Brassica oleracea L.var. capitata], one cauliflower [Brassica oleracea L.var. botrytis], and two red cabbage [Brassica oleracea L.var. rubra]) were assayed in terms of infestation rate and prevalence of plant pathogenic Xanthomonas Pseudomonas and Erwinia species and their identification. Totally 355 putative bacterial isolates were obtained from seeds and diseased sedlings developed from these seeds and identified by using traditional methods, host pathogenicity and serological (ELISA) methods. In examination of seedling tests, disease prevalence on seedlings developed from seeds under investigation varied between 5.8% and 51.6%. Results of identification of bacterial isolates revealed that seeds of Brassicaceous plants were not contaminated by Xanthomonas campestris pv. campestris, causal agent of black rot. Two different plant pathogenic bacterial species belongs to Erwinia and Pseudomonas genus were detected in different seeds lots.