Isolation and genotyping of campylobacter from broiler chickens

In this study, Campylobacter coli and Campylobacter jejuni isolation will be made after samples taken from the intestine and galls of Broiler Chickens slaughtered in a slaughterhouse. The factors isolated will be identified by using Polymerase Chain Reaction (PCR) method. In order to reveal the genetic difference between isolates, genotyping will be performed by means of Polymerase chain reaction - Restriction fragment length polymorphism (PCR-RFLP) method which is specifically applied to flagellin gene. Campylobacter species cause food infection and intoxication in humans and abortus in livestock. In order to combat effectively with infections generated by Campylobacters, the agent must be detected in a correct and quick way. In this study, the aim is to determine the colonization of Campylobacters in poultry with the help of methods to be used. In this study, during the slaughter from 100 Broiler Chickens, 200 samples, including 100 bile and 100 intestinal samples, will be collected. Under aseptic conditions in the lab, direct seeding without enrichment will be performed by using Blood Free Campylobacter Agar Base (CCDA agar). Cultures isolated because of Campylobacter suspicion, will be identified with Campylobacter jejuni and Campylobacter coli specific PCR method and cultures identified will be typed after determining polymorphism in the flagellin with PCR-RFLP method. keywords:Campylobacter, PCR, Broiler, RFLP.