Identification and characterization of Cauliflower mosaic virus (CaMV) isolates in Çanakkale province

In this study, a survey was conducted in Canakkale Province and sub province on cauliflower and cabbage plants that show Cauliflower mosaic virus-like symptoms between the years 2010-2011. A total of 84 samples were collected from these areas and tested by DAS-ELISA and/or PCR for the presence of CaMV. As a result of the DAS-ELISA and/or PCR analysis 63 out of 84 samples were found infected with CaMV. The coat protein genes of some isolates were cloned and sequenced. Isolates were further characterized and the sequences obtained from CaMV isolates of Canakkale were compared with known sequences from other part of the world to determine the genetic differences and evolutionary relationships among CaMV isolates from Canakkale and the other parts of the world. Comparison of CP genes revealed that CP gene of CaMV isolates from Canakkale Province and sub provinces showed 93-100 % and isolates with the world 92-97 % identity in their nucleotide sequence, respectively. Phylogenetic analysis of the CP gene sequences showed that CaMV isolates from Canakkale provinces and sub provinces displayed different level of genetic relationship with CaMV isolates from those of the world. In this case the method isolates proteins were analyzed by western blot analysis. As a result of western blot analysis of some isolates with 44 kDa, 39 kDa and 37 kDa weight proteins were obtained.