CCR2 C64I polimorfizmi ve CCR5 delta 32 delesyonu ile koroner arter hastalığı arasındaki ilişki

Inflammation plays an important role in the development and progress of atherosclerosis. Chemokines are small proteins secreted by certain cells to attract inflammatory cells such as macrophages, neutrophiles and lymphocytes to the inflammation site via their corresponding receptors. Ccr2 V64I polymorphism was shown to be involved in atherosclerosis in mice models. The human studies, however, found conflicting results. Ccr5 Delta 32 deletion results in a truncated protein that doesn't reach the surface and function as a receptor. This deletion was associated with coronary artery disease in animal and human studies. In this study we aimed to investigate the relationship between Ccr2 V64I polymorphism, Ccr5 Delta 32 deletion and significant coronary artery disease. Blood samples were collected before coronary angiography. Ccr2 V64I polymorphism and Ccr5 Delta 32 deletion was studied by PCR and then gel electrophoresis. Significance of coronary artery disease was evaluated by SYNTAX score. The patients were grouped into patient group ( with significant coronary artery disease) and control group (without significant coronary artery disease). Median age was 61 and majority of subjects were male (54.5%). Patient group had significantly more hypertension, diabetes, and hyperlipidemia. Prevalence of Ccr2 V64I allele was 30.7% in overall population and did not differ between patient and control groups. Prevalence of Ccr5 Delta 32 mutation was low (5.9%) and no statistical comparison could be made between groups. Subjects with Ccr2 V64I polymorphism had significantly higher diabetes whereas subjects with Ccr5 Delta deletion had significantly higher body mass index. Linear regression analysis revealed male gender, hypertension, obesity and low HDL as independent predictors of significant coronary artery disease. Our study adds valuable information about the role of chemokine system in atherosclerosis. Inflammatory pathways should be thoroughly investigated to unravel potential therapeutic targets and preventive measures.