A study into the changes in ALS1 adhesion gene levels in Candida albicans cells isolated from various soft lining materials in different time periods

The aim of this study was to investigate the quantitative expression of ALS1 gene mRNA isolated from the surface of the soft lining materials, after the incubation of four different soft lining materials subjected to a process of in vitro accelerated aging and of the ones not subjected with C. albicans cells for 12 and 24 hours.In this study, Visco Gel (room temperature curing acrylic based soft lining material), Vertex Soft (heat-cured acrylic based soft lining material), Dentusil (room temperature vulcanized silicone based soft lining material) and Molloplast-B (heat-vulcanized silicone based soft lining material) were used . 40 specimens from each material were prepared so as to obtain totally 160 specimens. Of 40 specimens, 20 were subjected to accelerated aging process by using Atlas UV2000 Accelerated Air Conditioning Test Device and the other 20 were not subjected to aging process. 10 of the 20 specimens which were subjected to accelerated aging were incubated with C. albicans for 12 hours and the other 10 specimens were incubated with C. albicans for 24 hours. And 10 of the 20 specimens which were not subjected to accelerated aging were incubated with C. albicans for 12 hours and the other 10 specimens were incubated with C. albicans for 24 hours. The biofilm layer occured on the surfaces of the specimens were excavated and mRNAs were isolated. The mRNAs were converted into cDNA and quantitative analyses were performed with real-time PCR.When the average gene expressions obtained from the in vitro accelerated aging process applied and not applied materials were evaluated, the least gene expression was found in Dentusil.The most statistically significant gene expression was observed in the Molloplast-B, when the average values of the materials were evaluated (p<0.05) .As a result of this study, we observed that the types of soft lining materials, duration of the material usage and the incubation period of C. albicans influenced the expression of ALS1 gene.Keywords: Candida albicans, adhesion, ALS1, gene expression, soft lining materials, accelerated aging