COVID-19 hastalarında MTHFR gen polı̇morfı̇zmı̇nı̇n belı̇rlenmesı̇

The Covid-19 pandemic denotes an exceptionally transmissible illness originating from the SARS-CoV-2 virus, a recent inclusion within the coronavirus lineage. It was first identified in December 2019 in Wuhan, a city located in the Hubei province of China. The rapid spread of this illness across international borders led the World Health Organization (WHO) to officially declare it a global pandemic in March 2020. In the scope of this academic research endeavor, our main objective was to examine the effects of genetic variations within the methylenetetrahydrofolate reductase (MTHFR) gene C677T in individuals affected by COVID-19. A cohort comprising 140 participants including 99 Covid-19 patients and 41 healthy control was procured from Tokat Government Hospital, subsequently transported to the Faculty of Medicine, and subjected to comprehensive analysis within the laboratory facilities of the Department of Medical Biology. The investigative procedures involved DNA isolation, employing a series of methodologies, including Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) techniques. The obtained results were subsequently visualized through Gel Electrophoresis, facilitating the identification of genetic variants. The primary objective of this analytical endeavor was to discern potential correlations between the Methylenetetrahydrofolate Reductase (MTHFR) gene C/T polymorphism (rs1801133) with the severity of Covid-19 manifestations with a p-value less than 0.05. Significantly, a discernible proportion of our results, precisely 42.86%, demonstrated statistical significance in the comparisons of genotypes between the Control group and patients, as well as between mild and inpatient cases in Turkish population. The calculated p-values for these respective genotype comparisons were 0.0154, 0.0222, and 0.0204.