An investigation on crossing possibilities in Cucurbita genus

In this study Cucurbita moschata, Cucurbita maxima and Cucurbita pepo species were crossed and observed which combination could produce seeds with germination ability.At the begining of the study pollens of each species were evaluated under SEM and noticed that; although sizes of pollen are different all species has same pollen type and shape.25-45-60-90-120 minutes after pollination; pistilles were taken in FAA and evaluated under SEM after staining by anilene and methyelene blue and detected that; pollen tubes entered the ovule 90 minutes after pollinationAfter crosses between C. maxima, C. moschata, C. pepo, species it was observed that C. moschata could be crossed with C. maxima and C. pepo and obtain fertile seeds but C. pepo and C. maxima crosses were failed from both way.C. maxima ? C. pepo crosses were repeated by cutting the stigmatic surface from 2-5 mm and then stored in FAA 4 hours after pollination. Pistilles of succesfull and unsuccesfull cross combinations were observed under floresence microscope and understood that; reason of infertile seeds obtaining from interspesific crosses between C. pepo and C. maxima was not due to incompatibility on stigmatic surface or in stilus.KEYWORDS: Cucurbits, Interspesific Cross, Bridge Cross, Formaline Acetic Acide Alcohol, Aniline Blue,