Molecular typing of Mycobacterium tuberculosis isolates from Çukurova area on IS6110 element polymorphism using RFLP analysis

Tuberculosis is one of the major public health issues in the developing countries of the world. In control of Mycobacterium tuberculosis, which has begun to increase together with the HIV epidemic in developed countries, specific diagnosis methods which is new, faster, cheap, sensetive and creation more effective anti-tuberculosis medicines, which can identify the public travel route and the transmission ways in genome level, epidemiological methods are required . In this study, isolated between 2007-2010 in Cukurova Region M.tuberculosis isolates by IS6110-RFLP typing method is aimed.In this study, 230 Mycobacterium tuberculosis complex strains isolated from the clinical materials of patients that have pulmoner tuberculosis or contact suspicious which were sent our laboratory from the different hospitals and Tuberculosis Dispansery in the Cukurova Region between the dates of 2007-2010 were used. Strains were evaluated with IS6110-RFLP typing method for clonal level diagnosis.Studied 230 isolates with IS6110 RFLP analysis of 146 different patterns were identified. One hundred and twenty samples contain 35 clusters (29.1%), 62 specific profile (26.9%), 36 strains were similar (15.6%) and 12 samples (5.2%) unidentified. According to this study, the rate of clustering was lower than results of other IS6110-RFLP studies done in our country.