Iron deficiency anaemia (iDA) and on the treatment of ida dna damage: 8-hydroxy-2-deoxyguanosine level

Iron Deficiency Anaemia (IDA) is a common and an important health problem in developing countries. Since, IDA -which lasts long on children- causes deterioration in growth and mental development, early diagnosis and effective treatments are important. Ionized form is potentially dangerous while the iron components which are in the form of inorganic components have an important duty. Ionic iron causes damages at the cellular level by forming free radicals. Therefore, decreases and increases in the level of iron have clinic significances. Reactive Oxygen Species (ROS) lead to the formation of oxidative base damages in DNA. Among these forms the most common one and the one which has the best known mutagenity is 8-hydroxy-2?-deoxyguanosine (8-OHdG). We aimed to determine IDA and its different forms of treatments? (p.o., i.m., i.v.); probable oxidative damage on DNA by looking at the level of 8-OHdG. In this way, we aimed to bring a new perspective to the treatment of IDA. The total number of patients studied was 80 and 60 of them had IDA and the rest were healthy control group. The patients were divided into 4 subgroups: (First group: Oral treatment (p.o.) group with 20 patients]; Second group: Intramuscular treatment (i.m.) group with 20 patients]; Third group: Intravenous treatment (i.v.) group with 20 patients]; Fourth group: Healthy control group 20 patients]. Blood and urine samples were taken from all patients totally four times; just before the treatment, at the twenty-fourth hour of treatment, at the first week of treatment and at the third month of the treatment. 8-OHdG levels detected in blood and urine samples were compared with the control group. Especially at the twenty-fourth hour of treatment and also at each stage, statistical significance high was detected at the 8-hydroxy-2-deoxyguanosine blood and urine level in i.v. group compared to other groups (p<0.05). Iron Deficiency Anaemia and the treatment of it affect the level of 8-OHdG. Oral iron therapy should be the top priority on children. If p.o. iron therapy cannot be applied, i.m. iron therapy can be preferable. Intravenous iron treatment might be chosen if the indications make a need. Key words: Anaemia, 8 hydroxydeoxyguanosine, oxidative damage, DNA damage.