Experimental myocardial infarction and irisin

ABSTRACT EXPERIMENTAL MYOCARDIAL INFARCTION AND IRISIN Our knowledge about Myocardial Infarction (MI) pathophysiology is still very limited. In this study, we aimed to investigate the irisin levels in the heart tissues of the rats with experimental myocardial infarction. In this study, 21 rats were used. 3 groups were performed as 7 animals in each group. During the test period of 14 days, control group did not received any treatment and decapitated under anesthesia at the end of the experiment. Rats in groups Myocardial Infarction (MI-I) and Myocardial Infarction II (M-II) received 150mg/kg isoproterenol subcutaneously, 2 times with 24 hours interval. After the MI induction, rats in MI-I group were decapitated under anesthesia at the end of 1 week and MI-II group was decapitated at the end of 2 weeks. From removed heart tisues irisin levels were measured by Polymerase Chain Reaction (PCR) and immunohistochemical methods. In Masson trichrome staining, MI-I and MI-II groups revealed a striking increase in the connective tissue compared to the control group. According to the results of PCR; irisin levels of MI-I group were 23.3 +/- 0.2 times (p = 0.001) and MR-II group were 15.93 +/- 0.3 times (p = 0.001) higher in comparison with control group. Difference between MI-I and II groups was not statistical significant (p> 0.05). In IHC study, the severity and extent of irisin immunoreactivity increased in both MI-I and II groups in comparison with control group (p = 0.01). Difference between MI-I and II groups was not statistical significant (p> 0.05). According to the results of this study, it is possible to say that irisin has important role in the pathophysiological mechanism of MI. More detailed future studies will show the role of irisin clearly. Keywords: Acute myocardial infarction, irisin, isoproterenol.