Investigation of voltage dependent anion channel-1 and hyperphosphorylated tau inhibition in an experimental alzheimer's disease model

Alzheimer's Disease (AD) is defined as a primary neurodegenerative disease of the brain, which has an idiopathic etiology, causes characteristic neuropathological and neurochemical changes, has an insidious onset, lasts for years and progresses slowly. In this study, it was thought that inhibiting the pro-apoptotic activity of VDAC1 might be an ideal method to inhibit cell death in AD. The study, it was aimed to examine the relationship between the inhibition of voltage-dependent anion channel protein known as gatekepeer and davunetidine treatment on primary pathological markers of AD and their ability to recover memory-memory deficits in the AD model. A total of 30 male wistar rats weighing 230±30 g and 2-3 months were allocated. 5 animals in each group were randomly divided into 6 groups as CONTROL group, SHAM group, STZ group, STZ-DAV group, STZ-DIDS group and STZ-DIDS-DAV group. Intracerebroventricular streptozotocin was administered for the AH model. During the experiment, DIDS and DAV applications were performed intraperitoneally. The radial arm maze (RAM) test was used in behavioral tests. After 21 days of administration, blood and brain samples were taken from rats under anesthesia. Then, biochemical analyzes were performed by ELISA method. While no difference was observed between the groups in terms of TNF-α, caspase-3 and MAPt values in serum, a significant difference was found in serum IL-10, TBARS, Aβ1-42, TSPO and VDAC1 values. While no significant difference was observed between IL-10 and Aβ1-42 values in brain tissue homogenates between groups, a significant difference was observed between TNF-α, TBARS, caspase-3, MAPt, TSPO and VDAC1 values. Differences were found between the RAM test data and the pre-training days and the training days. The fact that these applications can be effective in recovering memory-memory deficits on biochemical and behavioral data in rats with experimental AD model should be supported by further multi-faceted studies. Experimental AD model in rats, it should be supported by further multidimensional studies that these applications can be effective in recovering memory - memory deficits on biochemical and behavioral data.