Determination of anticancer and antioxidant activites of salvia aramiensis on lung cancer cells

This study was carried out to investigate the phytochemical content, antioxidative and anticancer effect of S. aramiensis from Lamiaceae, which is a medically important family. For this purpose, the plant obtained from its natural growing area were extracted by using methanol. As a result of LC-MS analysis of the plant extract, 5 different compounds determined. In antioxidant activity tests, the DPPH and metal chelating effects of MeOH extract of S. aramiensis were investigated. As a result of that, weak antioxidant effect of S. aramiensis was observed. Antiproliferative effect of S. aramiensis was examined and observed that lung cancer cells decreased at doses of 100 (p<0.005) and 200 (p<0.0001) µg/ml. IL6, IL8 and Caspase3 levels were measured by ELISA method. While both IL6 and IL8 levels increased at 25, 50 and 100 µg/ml doses, a significant decrease was observed at 200 µg/ml. Also, caspase 3 levels increased at 25 and 50 µg/ml doses and decreased at 100 and 200 µg/ml doses. Consequently, it was determined that the antioxidant activity of S. aramiensis is weak, the antiproliferative effect is strong, and IL-6 and IL8 can be reduced at high doses. However, it is thought that the cytotoxic effect may be due to a caspase-independent system due to the sudden decrease in caspase3 levels at 100 and 200 mg/ml doses Keywords: Salvia aramiensis, Phenolics, Antiproliferative, Antioxidant, Lung Cancer