Investigation of the components of deve çökerten (Tribulus terrestris L.) by different extraction methods

In this study, the effects of Sub-critical water extraction (sbcH2O) and Sub-critical Ethanol extraction (sbcEtOH) methods were investigated. These methods were compared in order to determine the chemical composition of the plant which has systematic name is Tribulus terrestris L.. In addition, the effects of (USE) Ultrasonic Asisted tert-butylmetyl ether extraction and maceration with tert-butylmetyl ether were investigated. These methods were compared. Besides, the biological activity determination of the extract of Tribulus terrestris L. with sbcH2O and the total nitrogen (protein) determination by Kjeldahl Method in Tribulus terrestris L. extract were investigated. Overall in this study in sbcH2O extraction 1.86 % Loliolide and 10.75 % Loliolide's derivative were observed. In the USE, 1.49 % of Loliolide and 50.30 % of Oleamide were determined. İn total, 0,20 % of Loliolide and 6,62 % of Oleamide were determined in maceration. According to the biological activity determination of Tribulus terrestris L. extract, the antifungal study on Candida albicans yeast was positive. A zone with a diameter of 22 mm has been formed. According to the total nitrogen (protein) determination by Kjeldahl Method, 14.47 ± 1.73 % protein was identified in the plant.