Development of spectrophotometric methods for the determination of amino drug compounds in pharmaceutical preparatio ns by molecular absorption. UV-VIS spectrophotometer

For the determination of drug and its quantity in pharmaceutics, there was a eed of development of new, highly sensitive, and effecient approach. İn this study, spectrophotometric method was developed for sodium diclofenic and thiamin hydrochloride. Both water soluble drugs give maximum absorbance at 600 nm and 441 nm of wavelength. İt was found that, developed method is highly sensitive and obtained final product is stable for long time to get enough measurment to determine its presence and quantity precisely. İt was found that, we can detect sodium diclofenic in the range between 0.8-16 µg/ml and thiamin hydrochloride 2.4-60 µg/ml accurately, as the developed method obey the Beer's Law. The molar absorption values, the sandell sensitivity index of 2.8756 Lmol-1 cm-1 and 0.0110 µg.cm-2 for sodium diclofenic and 8.526103 lmol-1cm-1 and 0.0390 µg/ml for thiamin hydrochloride was measured. The developed method was successfully applied for the determination of diclofenac sodium and thiamin hydrochloride in pharmaceutical preparations (pharmaceutical tablets and capsules) with reproducibility ranged of 98.4-99.9 %.