Structure of collagen and blood vessels of ovarium, in teenage and old, in diabetic and normal pregnant rats

Diabetes is a metabolic disorder resulted from rising of blood glucose level extremely. Pregnancy and diabetes are cases affecting each other negatively. It includes aging that is deterioration process affecting cell, tissue, organ and systems.In experiment models created in our study, it was aimed to be examined the collagen content belonging to ovary that is known underwent various changes in pregnancy, diabetes and senility and effects to be created by every three cases together in vein structures, histological changes and damage that are probable to develop immunohistochemically and comparatively by using collagen type III, IV, VI and alpha actin primary markers.In our study, total 48 Sprague Dawley species female rats were used; 24 rats are 2 months old (young) and 24 rats are 8 months old (old). Totally 8 groups were created in such a way that there would be 6 rats in each group. Experimental diabetes was created by applying one dose 50 mg/kg STZ with intraperitonal injection in rats included in diabetes group. In subjects determined that they had diabates after 2 months' follow-up period following the implementation, pregnancy was provided. In 14th day of pregnancy, ovaries belonging to all groups were removed. Immunohistochemical results were evaluated in light microscope level.A clear reduction was found in type collagen types III, IV, VI in diabetic and pregnant groups. It was seen that old and diabetic group ovary tissues of said markers had the least expressions. Contrary to this, an increase was observed in expression of type IV collagen and alpha actin expression in vein structures of diabetic, pregnant and old groups.