Investigation of uPAR and vitronectin moleculs in endometrium of diabetic rats

Summary: We aimed to investigate the expressions of the uPAR and vitronektin molecules immunhistochemically in STZ diabetic rats uterine tissues lumen of the epithelium, glandular epithelia and stroma before the proeustrus and eustrus preimplantation periods in this study. In addition, examine the histomorphologic changes that occurred before the implantation process in diabetic uterine by histochemical and ultrastructural techniques. Objective: 28 wistar female rats were used in this experiment. Animals were divided into 4 groups by using vaginal smear method and the rats which were in the convenient phase were sacrificed. Group 1 (control group): This group of which uterus tissue samples were taken in phase of proeustrus of cycle by applying nothing. Grup 2 (control group): This the group of which uterus tissue samples wer taken at phase of eustrus of cycle by doing nothing. Group 3(diabet group): This is the group of which uterus tissue samples were taken at the phase of proeustrus 8 weeks after applying STZ (dissolved in sodium sitrate) given intraperitoneally which. Group 4 (diabet group): This is the group which uterus tissue samples were taken at the eustrus phase 8 weeks after applying STZ (dissolved in sodium sitrate) which was given intrapritoneally. All rats were sacrifeced 8 weeks after applying the experiment. For the histochemical investigation, hematoxylin eozin (H&E), Masson Tricrom, periodic asit shiff (PAS), stainings were done with vitronectin and uPAR antibodies for immunohistochemical staining. Thin sections were taken for ultrastructural examination. Result: As a result of investigations, immunohistochemical distribution of vitronectin molecules in diabet proeustrus group reduced when comparison to control group. In diabet proeustrus group immunpositive vitronectin molecules increased in comparison with control group. As for uPAR molecules in diabet proeustrus group, immunpositivity increased when compared to control group, in diabet eustrus group increase of uPAR immunpositivity seen in comparison with control group. Conclusion: Depending on the daha we obtained; we observed that diabet causes uterus tissue damage in the period of preimplantation and molecules of vitronectin and uPAR were necessary for implantation in uterus tissue. Key words: Diabetes, proeustrus, eustrus, vitronectin, uPAR