In vitro evaluation of antimicrobial effects of natural sourced products on oral pathogens

In this thesis; antimicrobial efficiency of stone water (Kale Natural, Balıkesir, Turkey), pine turpentine essence (Ali Ünyazıcı, Ankara, Turkey), olive leaf extract (Kale Natural, Balıkesir, Turkey), grape seed oil (Ali Ünyazıcı, Ankara, Turkey), coconut oil (Ali Ünyazıcı, Ankara, Turkey), propolis (Bee'o Up, Istanbul, Turkey), tea tree oil (Ali Ünyazıcı, Ankara, Turkey), pine bark extract (Kale Natural, Balıkesir, Turkey), 2% chlorhexidine (Drogsan, Ankara, Turkey), glycerol and distilled water on Enterococcus faecalis (E. faecalis, ATCC#29212), Streptococcus mutans (S. mutans ATCC#25175), Lactobacillus acidophylus (L. acidophylus ATCC#4356) and Candida albicans (C. albicans ATCC#10231) were investigated. Disc diffusion and well diffusion methods were repeated three times, and microdilution test was studied with natural products that showed efficacy. Among the groups formed by natural products, preparations with antimicrobial effect and chlorhexidine digluconate group were included in the statistical analysis. The conformity of the data to the normal distribution was examined by the Shapiro-Wilk test and the compatibility to the homogeneity assumption with the Levene test, the Kruskal Wallis test was used as the analysis method. Dunn's test was used as an advanced test in order to reveal the groups with differences. The p<0.05 criterion was taken as a basis in the evaluation of the mentioned statistical methods, and SPSS 26 and R Studio package programs were used in the application of the tests. As a result of the study, pine turpentine, propolis, tea tree oil and red pine bark extract; showed antimicrobial activity against oral pathogens used. Tea tree oil showed the highest antimicrobial activity against C. albicans and was significantly more anticandidal than 2% chlorhexidine digluconate. Although pine turpentine, propolis, tea tree oil and red pine bark extract showed activity against L. acidophylus, it did not show antibacterial properties as high as 2% chlorhexidine digluconate. It was concluded that natural products are as antimicrobial as chlorhexidine against S. mutans and E. faecalis.