Doku mühendisliği uygulamaları için antibakteriyel, biyobozunur ve biyouyumlu malzeme üretimi

Tissue engineering aims to restore, maintain, or improve damaged tissue functions. Cells, biochemical factors, and scaffolds are referred to as the key components of tissue engineering. The scaffold has a crucial role in tissue regeneration process by providing a microenvironment for cell attachment, proliferation, and differentiation, and new tissue formation by mimicking an extracellular matrix. This thesis aimed to produce biodegradable and biocompatible materials for bone and cartilage tissue engineering as well as wound healing applications. In the bone tissue engineering part, three dimensional and porous hydroxyapatite (HAP) incorporated and silk impregnated poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) nanofibrous cotton wool-like scaffolds were fabricated by wet-electrospinning followed by freeze-drying techniques. The produced scaffolds exhibited improved bioactivity and biomineralization tendency and therefore, it can be evaluated as a candidate filling material for bone regeneration. In the cartilage regeneration part, PHBV nanofiber reinforced carboxymethyl chitosan (CMCht) - silk hydrogel scaffolds that were cross-linked by poly (ethylene glycol) diglycidyl ether (PEGDE) were prepared. The designed scaffolds demonstrated good properties in terms of damping capability, thermal stability, compressive strength, and chondrogenic differentiation as an indication of being a promising material for cartilage tissue regeneration. In the wound healing part, modified cotton hydrogel wound dressings were produced by antibacterial nisin incorporation and CMCht-alginate impregnation. It was concluded that the produced dressings would be used as wound dressings for acute wounds due to the fact that they retained their porous structures after modification, had high water up-take capacity, exhibited viscoelastic properties and antibacterial activity against gram-positive Staphylococcus aureus bacteria.