Investigation of docetaxel and deguelin's chemopreventive activity in lung cancer cell line

INVESTIGATION OF DOCETAXEL AND DEGUELIN'S CHEMOPREVENTIVE ACTIVITY IN LUNG CANCER CELL LINE ABSTRACT Despite intense efforts to prevent and treat cancer, cancer is one of the most commen causes of death. In cancer-related deaths, lung cancer is ranked first in men and women all over the world. The most effective cancer treatment agents are also toxic to normal tissues. For this reason, new, preventive and curative medicines with very low or no toxicity are needed. This study was based on the hypothesis that Deguelin in effective concentration has an inhibitory effect on the proliferation of neoplastic cells in lung cancer cell lines and DEG+ DTX combination is more successful than single usage of the drug. This study examined the anticancer effect of Deguelin on H1299 and A549 lung cancer cell lines. In this study, pure Docetaxel and Deguelin cytotoxic effects on A549 and H1299 lung cancer cell lines were investigated. IC50 doses were determined. At the determined effective concentrations, combinations of Deguelin and Docetaxel were applied to lung cancer cells. Flow Cytometric Analyzes (Apoptosis, cell cycle), Migration analysis and Colony Formation Test were performed in cell cultures. In our research; It has been observed that the DEG+DTX combination is more successful in proliferating lung cancer cells than drugs used alone, Deguelin's anticancer effect could be by reducing the spread of cells, rather than leading to the death of cancer cells. Key words: Lung cancer cell lines, Deguelin, Docetaxel, IC50, apoptosis, cell cycle, number of colon, cell migration