The effect of gold nanoparticles synthesized by green synthesis method on GSK-3 beta mRNA expression in human neuroblastoma cell line

As nanotechnology is one of the most promising era of many fields such as electronic field power field and health care and medicine field as many other field. In-depth research and biomedical applications such as genomics, biosensors, immunoassays, clinical chemistry, laser phototherapy of cancer cells and tumours, targeted drug delivery of drugs, DNA and antigens, optical bio imaging, and the monitoring of cells and tissues with the use of cutting edge detection systems, are all focused on gold nanoparticles with controlled geometrical and optical properties. Plant generated AuNPs represent a great opportunity to be used in many field especially the medical field such as drug delivery and therapy, which those nanoparticles synthesised by green synthesis have a high antioxidant and anti-inflammatory quality as well their permeability that they can cross many biological barriers such as blood brain barrier, which is a very specific and selective barrier as it protect the brain from the foreign molecules, as well control what can pass and what couldn't to the central nervous system. Thus, they can be used for drug delivery in many neurodegenerative diseases. As signalling and metabolic enzyme, glycogen synthase kinase 3 beta (GSK-3β) participate in a number of biological functions, including signalling, cell cycle regulation, DNA repair and proliferation of cells. Many disorders such as cancer, diabetes, neurological disease, and inflammation have been linked to GSK-3 beta. The primary objective of this study was be to examine the effect of gold nanoparticles produced by the green synthesis method on the expression of mRNA of GSK3β in SH-SY5Y human neuroblastoma cell line. We used both MTT and qPCR to see the effect of the gold nanoparticles on the gene express of GSK3β as well its toxicity on our cells and observe the ideal concentration of gold nanoparticle in terms of its biocompatibility. As a result, 10 μg/μl AuNPs was shown to be the most biocompatible concentration in SH-SY5Y human neuroblastoma cell and this concentration of AuNP increase the gene expression of GSK3β.