Significance of rapd method for the detection of filogenetic relation between virulance factors identified E.coli strains isolated from extraintestinal infections

The importance of RAPD method in the investigation of filogenetic relation of E.coli strains isolated from Extraintestinal infections and virulence factors of which are determined. E. coli strains which are the commensal members of intestinal ecosystem and which are adapted to any environmental condition due to its great recombination mutation ability have not only high potential of GC but also an interesting genomic plasticity feature. In addition to these features, E. coli which can rapidly grow in classic medium is a good model accounting for DNA transfer cases. In fact, they may gain new virulence features which do no exist in them with the gene transfer in which proteins playing a role in the adaption of microorganism to any micro environment, yet providing microorganism with different virulence are coded. Whether intestinal ExPEC strains are developed as a result of special genotype mutation or random mutation developed ExPEC strains is of great importance for the tracking the signs of these strains in the society and for the infections control protocols. Thus instead of together of with fenotypic methods which have a low level of differenciation such as clinical patotypes, physiological features multilocus enzyme electrophoresis phylotypic, such methods as PCR, PCR-RFLP, PFGE and Multilocus Sequence Typing (MLST) sequencing which are based on nucleic acid amplification and can determine specific sequence and/or in which restriction enzymes are used are needed. In this study; We aimed at investigating the determination power of RAPD and XbaI-PFGE in which three different short oligonucleotide sequences were used in order to determine the possible clonal relation ExPEC isoates some virulence genes of which were shown in the the strains isolated from extra intestinal system. In the study, 155 ExPEC strains isolated from various clinical samples were used. Key words ExPEC, CDT, CNF, PFGE and RAPD