The time course effect of exogenous egf administration on oxidative stress of submandibulary gland

viTHE T ME COURSE EFFECT OF EXOGENOUS EGFADMINISTRATION ON OXIDATIVE STRESS OFSUBMANDIBULARY GLAND(M.Sc. Thesis)Seda TAŞIRGAZ UNIVERSITYINSTITUTE OF SCIENCE AND TECHNOLOGYJanuary 2007ABSTRACTEpidermal growth factor (EGF) is found in high concentrations insubmandibular gland (SMG) and saliva. Various growth factors such as EGFplay significant roles during wound healing process. There are limited numbersof studies on the effect of exogenous EGF implantation on oxidative events ofsalivary gland. That is why we planned to investigate the effects of oralsubmucosal implanted EGF on the oxidative stress of salivary gland.In this study we used New Zeland albino rabbits (male, n=43, 2,5 ± 0,4 kg). Aftersubmucosal incisions were made the rabbits were divided into two equal groups:1- Untreated wounds 2- EGF implanted wounds. The rabbits were killed byexcess of sodium penthobarbitale anesthesia on the 1st, 3th and 5th days ofpostwounding. Submandibular glands were excised immediately. The levels ofthiobarbituric acid substances (TBARS), last products of lipid peroxidation,glutathione (GSH) and ascorbic acid (AA), important antioxidants, and theactivity myeloperoxidase (MPO) enzyme that takes role in oxygen-mediated hostdefence mechanisms were measured by spectrophotometrically. Results werecompared by Anova Variance Analysis and Mann Whitney U test.In conclusion, exogenous EGF treatment caused a decrease in TBARS levels ofsalivary gland and increase in MPO activity of salivary gland because of itsviipositive effects on wound healing. GSH levels of salivary gland were not changed.And finally, AA levels of salivary gland were decreased.Science Code : 203.1.057Key Words : EGF, salivary gland, lipit peroxidation,antioxidant, MPO, Vitamine CPage Number : 88Adviser : Doç. Dr. Şule COŞKUN