Determination of the efficiency of intracervical and laparoscopic insemination with frozen semen in intensively grown halep goats after PGF2 alpha and progesterone based estrous synchronization in breeding season

In this study, the effectiveness of different oestrus synchronization methods in Aleppo goats during the breeding season and the effect of insemination time and insemination technique on fertility in artificial insemination applications with frozen semen after synchronization with intravaginal sponges containing synthetic progesterone were evaluated. In the research, 160 goats aged between 2-4 and without fertility problems and 12 bucks aged between 3-4 years were used for estrus detection. Frozen-thawed Saanen goat semen straws were used for artificial insemination. Herbal progesterone (BP), herbal progesterone+eCG (BP+eCG), double dose PGF2α (PG), double dose PGF2α+eCG (PG+eCG), and synthetic progesterone (SP+eCG+PG; control) in trial I. In trial II, MAP vajinal sponges were applied to all of the goats in the treatment groups for 11.5 days, and eCG and PGF2α injections were made at the end of the application. Goats were inseminated by intracervical and laparoscopic methods based on a fixed time or oestrus monitoring. In trial I, the lowest values in terms of oestrus rates were found in the BP (35%) and BP+eCG (40%) groups, and the difference between the groups was significant (P<0.05). In the BP, BP + eCG, PG, PG+eCG, and SP+eCG+PG (control) groups, the control, and PG groups were the highest on the 21st day in terms of plasma P4 levels on the 21st day following insemination and pregnancy rates according to the ultrasonographic examination on the 50th day, BP+eCG group was the lowest; on the 50th day, while the control group was higher than the PG+eCG and BP+eCG groups, the lowest pregnancy rate was recorded in the PG and BP groups (P<0.05). In Trial II, in terms of pregnancy rate, inseminations were performed with intracervical and laparoscopic methods at a fixed time and 12-16 hours after estrus; The highest pregnancy is 12-16 after insemination with the laparoscopic method and after the determination of estrus. As a result, the highest success in terms of oestrus rate from different oestrus synchronization protocols was found in PG, PG+eCG and SP+eCG+PG (control) groups, while the lowest oestrus rates were seen in the groups that were administered natural herbal progesterone. It was determined that the highest rate in terms of pregnancy rate was obtained with the laparoscopic method performed 12-16 hours after the detection of estrus. Keywords: Goat, estrus synchronization, frozen semen, intracervical, laparoscopic, artificial insemination.