Investigation of Human Leukocyte Antigen-G molecules expressing on B Lymphocytes infected with Epstein-Barr Virus

Denoting less polymorphism when compared to classical Human Leukocyte Antigen class I (HLA class I) molecules, the Human Leukocyte Antigen-G (HLA-G) molecule has 7 different isoforms. Initially, HLA-G was considered to be expressed in trophoblastic cells but it was later on proved to have permanent expression in immune privileged sites and to have determinable soluble forms. HLA-G is known as a molecule that causes immune suppressionEpstein-Barr Virus (EBV) is associated with several malignant tumors besides being a persistent and common agent of infection. Either immune expression or immune response incompetence of the immune system against EBV has a major role in EBV?s persistenceThe aim of the study was to search expression variance of the HLA-G molecule on the surface of B cells dependent on its transfection with EBV, time and presence / absence of CD2+ cells and to search variance of soluble HLA-G level. To this end, B95-8 cell source existent in cell bank of Gazi University Head Department of Immunology which produces EBV virion has been used in the transfection of B lymphocytes. In the presence and absence of CD2+ cells and in the presence and absence of EBV transfection; 24, 48 and 72-hour cultures have been prepared and HLA-G expression on the surface of cells have been measured by flow cytometry by using monoclonal antibodies and soluble HLA-G (sHLA-G) levels, interleukin-10 (IL-10) and transforming growth factor-beta (TGF-ß) levels have been measured by ELISA methodIn respect of the study results, HLA-G expression on the surface of CD19+ cells was determined as independent from EBV transfection yet showing a time related increase in the absence of CD2+ cellBy the results it reveals, the project study shall inherently be quoted as authority for literature and further studies.