Effects of pharmacologic agents on heart rate variability and complexity in mice

frequency-based) and non-linear heart rate fluctuations that may occur with pharmacological agents (Flecainide and Isoproteranol). In this way, the effects of agents such as Flecainide and Isoproteranol on the autonomic nervous system that determines the heart rate will be tried to be understood. Material and Methods: In our study, 24 male mice aged 4-6 months were used to investigate the cardiac dynamics of the autonomic nervous system. They were divided into 3 groups (n=8): 1. Serum Physiological (SF), control, 0.4 ml (i.p), 2. Flecainide 20 μg/g, 3. Isoproteranol 4 μg/g. For anesthesia, 75 μg/g Na-pentobarbital + 0.04 μg/g Fentanyl combination was applied to all mice i.p. ECG records were taken before and after drug administration in all groups. In order to perform the heart rate variability analyses, which is the main subject of this study, the number sequence of spontaneous breathing frequency was obtained with LAB Chart 7 software. The R-R interval sequence was analyzed with KUBIOS software (FINLAND) and the heart rate variability analyses were completed. Results: ECG data was affected as expected in Flecainide and Isoproteranol groups. QJ interval decreased in ISO compared to FLEC. However, there was no significant difference in time and frequency parameters. SDNN and RMSSD were not significant in nonlinear parameters. However, it was observed that ISO decreased in DFA1 value compared to both FLEC and SF. Conclusion: In Flecainide and Isoproteranol groups, ECG data were almost close to literature data. However, no significant results were found in HRV. The reason for this not being significant is that the standard deviations of the data were very large. Only the DFA1 value was significant in nonlinear parameters. DFA1 reflects more parasympathetic because it is short-term. This means that it suppresses complexity.