Investigation of the effect of different exercise applications on irisin, heat shock protein 70 and some biochemical parameters

2. ABSTRACT Investigation of the Effect of Different Exercise Applications on Irisin, Heat Shock Protein 70 and Some Biochemical Parameters The aim of the study was to determine the effects of different and regularly applied exercise programs on body composition, irisin, heat shock protein 70 and some biochemical parameters. 120 male university students voluntarily participated in the study, who were in the same age group. Participants were divided into 4 equal groups as control (C), resistance exercise group (RE), high intensity interval (HIT) and aerobic exercise group (AE). While the control group did not perform any exercise, the pre-determined exercise programs were applied to the other groups for 8 weeks and 3 days in a week. Blood samples were taken from all participants before the exercise program was started and after the exercise program was ended and body composition (fat-free body weight (FFBW), body mass index (BMI), total body fluid (TBF) and body fat percentage (BFP) analyzes were performed. Cholesterol, HDL and LDL cholesterol, triglyceride, creatine kinase (CK), lactate dehydrogenase (LDH), Irisin and Heat shock protein 70 (HSP70) levels were analyzed in blood samples. SPSS package program was used in the analysis of the obtained data. After testing the normality of the distribution, Paired Samples t test was used to determine intra-group differentiation and One Way Anova test was used to determine the difference between the groups. The level of significance was accepted as p<0,05. As a result of the analysis; it is determined that there are significant differences in Pre-post test values of the aerobic exercise group's body weight, FFBW, LDH, cholesterol, HDL, Triglyceride and HSP 70 levels, high intensity interval group's BFP, FFBW, BMI, CK, LDH, Cholesterol, HDL, Triglyceride, Irisin and HSP70 levels and resistance exercise group's BFP, FFBW, TBF, CK, LDH, Cholesterol, LDL, Triglyceride and Irisin levels (p<0,05). There were significant differences between the groups in terms of BFP, FFBW, LDL, Triglyceride, CK, LDH, Irisin and HSP70 values (p<0,05). According to the obtained results it can be said that; Exercise can correct abnormalities in body composition and lipid profile, changes in HSP70 levels may vary depending on muscle damage, the increase of irisin due to exercise, activates fat metabolism and supports the positive results of exercise in terms of lean mass. Keywords: Exercise, İrisin, HSP70, Biyochemistry, Muscle damage