Isolation of Bacillus sp. from different sources and determination of biochemical characteristic

The aim of this research was the isolation of thermophilic Bacillus sp. from different regions and grouping by 16S-ITS rDNA RFLP. 74 thermophilic strains were isolated from various environmental samples. Since 15 isolated strains were Gram (+), endospore forming rods, they were identified as Bacillus sp. These strains were screened for the existence of 5 extracellular enzyme activities. These were, lipases, amylases, proteases, xylanases, cellulases, Some other phenotypic tests were also performed for these isolated strains. 16S-ITS rDNA RFLP profiles were produced by using two restriction endonucleases Taq I and Hae III. The isolated strains were clustered into 8 groups by Taq I restriction profiles of 16S-ITS rDNA while 7 groups were obtained by Hae III digestion profiles.Key words: Bacillus, 16S-ITS rDNA RFLP, extracellular enzyme, identification