Identification of pigment producing bacteria isolated from different sources and use as dyestuff in textile industry

In this study, the isolates that were isolated in the previous studies in culture collection were evaluated in term of pigment production. The isolates producing pigments were identified by classical and molecular methods. The pigments were isolated from bacteria by using methanol extraction method and characterized by TLC and spectrophotometer. The pigments were evaluated as dyestuff on silk fabric in textile industry. Besides their effects on human health were searched by MTT test on healthy fibroblast cell line and hemolytic activity test on erythrocyte cells. As a result of the study, 6 isolates were determined to produce the best pigment from 570 isolates. The isolates coded as AS-54, AS-55, AS-67 were identified as Serratia plymuthica, the isolate coded as AS-75 was identified as Serratia marcescens, the isoate coded as AHS was identified as Micrococcus luteus, the isolate coded as FAS-TUR was identified as Paracoccus marcusii. As a result of the characterization of the pigments, the pigments were found to be beta-carotene and prodigiosin for the isolates coded as AHS, FAS-TUR isolates and the others, respectively. At the end of the process of silk fabric dyeing, the colour of the fabrics were determined as different shades of pink, orange and yellow. The pigments did not exhibit any hemolitic activity and toxic effect on fibroblast cell line.