The Effect of AC electric field with different application times on the protein synthesis

The effects of Extremely Low Frequency (ELF) Electric Field which we are exposed in daily life on collagen synthesis was studied in different exposure periods. The effect was evaluated by assessing the amount of hydroxyproline in the liver tissue. 1.35 kV/m AC electric field with 50 Hz frequency was applied to 40 guinea pigs in 4 different application times being 1 day, 3 days, 5 days and 7 days with daily exposure period of 9 hours. 15 guinea pigs were also kept in the same laboratory conditions and served as control without any electrical field application. At the end of each exposure period liver hydroxyproline contents were determined using Stegemann-Stalder's method. The applied electric field was found decreased hydroxyproline amount of liver tissues significantly in all of the exposure periods vv.r.t the controls suggesting decreased synthesis of collagen under ELF Electric field. Beside that, theoretical values of electric field, current intensity and current density on the surface and inside of the guinea pigs and human models were calculated.