Simultaneous determination of dexphantenol, mepyramine and lidocaine in combined pharmaceutical gels by capillary electrophoresis

A new capillary electrophoresis method was developed to analyze pharmaceutical preparations containing ternary combination of dexphantenol, mepyramine and lidocaine simultaneously. Best result were obtained by use of 20 mM pH 3,0 phosphate buffer as the background electrolyte. The separation was performed through a fused-silica capillary (75 µm internal diameter, 50 cm total length, 41 cm effective length) at 20oC with the application of 5 seconds of hydrodynamic injection at 50 mbar pressure and potential of 30 kV. Detection wavelength was 200 nm. Under these conditions, the migration times were found to be 7.363 min for dexphantenol, 2.457 min for mepyramine and 3.520 min for lidocaine. Linearity ranges for the method were 25- 200 µg/mL for DEK, 7,5- 60 µg/mL for MEP and 7,5- 60 µg/mL for LID. Limit of detection values were found as 3,06µg/mL for DEK, 0,76 µg/mL for MEP and 1,75 µg/mL for LID. According to the validation study, it was proved that the developed method was accurate, precise, sensitive, specific and repeatable.