Investigation of the effects of phenethyl isothiocyanate in fatty liver disease induced by HİGH-fat diet and streptozotocin in rats

Aim: In this study, it was aimed to investigate the effects of phenethyl isothiocyanate on the SIRT1-Nrf2 pathway against oxidative stress and liver damage in fatty liver disease caused by the induction of experimental diabetes induced by high fat diet and streptozotocin. Materials and Methods: 30 Wistar Albino rats (150-250g, 6-8 weeks old, male) were divided into three groups (each of 10 rats): control, high fat diet+ Streptozotocin (35mg / kg), high fat diet+ Streptozotocin (35mg / kg) + Phenethyl isothiocyanate (40mg / kg). Levels of serum ALT, and serum lipids (TG, TC, HDL, and LDL / VLDL), activities of antioxidant enzymes (SOD, CAT, GPx), and levels of MDA, GST, SIRT1, and NF-ƙβ, also activity of DNA binding levels of Nrf2 were determined. Histological examinations were performed on the liver tissues. Results: Compared to the control group, in the rats of the HFD+STZ group, serum HDL level, hepatic antioxidant enzyme activities (SOD, CAT, GPx), levels of GST, SIRT1, and NF-ƙβ, as well as DNA binding activity of Nrf2 decreased, serum ALT, serum lipids (TG, TC, LDL / VLDL) and MDA levels were observed to increase (p <0.05). It was determined that there was an improvement in these parameters in the group of PEITC treatment (p <0.05). Histological examinations supported biochemical analysis (p <000.1). Conclusion: PEITC improved NAFLD induced by HFD and STZ through the SIRT1-Nrf2 pathway. We can say that PEITC could be a promising and effective candidate agent against NAFLD. Key words: Phenethyl isothiocyanate, Nrf2, SIRT1, Streptozotocin, High fat diet.