Preparation, characterization and determination of antioxidant and antialzheimer effects of ferulic acid loaded PLGA-based nanosystems

Nanoparticle systems have attracted attention by researchers in recent years due to their advantages such as modified release feature, increasing therapeutic efficacy and reducing side effects. Ferulic acid has therapeutic effects such as anti-inflammatory, anti-alzheimer's, antioxidant, antimicrobial, anticancer, antihyperlipidemic, antidiabetic. In this study, ferulic acid loaded PLGA-based nanoparticles were prepared by nanoprecipitation method and the effect of varying concentrations of Poloxamer 188 and Span 60 on nanoparticle properties was investigated. A-FA coded formulation loaded with ferulic acid was chosen as optimum. High encasing efficiency has been achieved due to the low affinity of ferulic acid to the water phase and therefore its lipophilic nature, which tends to migrate to the organic phase. It was determined that the release of ferulic acid from the formulation coded A-FA was slower than pure ferulic acid and prolonged release in 24 hours. Antioxidant and antialzheimer's effects of A-FA coded nanoparticle formulation were investigated by biological activity studies. A-FA coded nanoparticle formulation showed strong DDPH free radical scavenging, ABTS●+ cation decolorizing and reducing antioxidant activity. Since it has both AChE inhibitor and antioxidant properties according to the results of its anti-alzheimer activity, it was concluded that the formulation prepared in this thesis study shows promise in the treatment of both oxidative stress-related diseases and Alzheimer's.