Fibrinolytic enzyme production

In this study isolation, identification and optimisation of protease enzyme producing strains from fermantative protein resources was investigated. The protease enzyme activity of the isolated strains was determined by analyses performed on solid and liquid enzyme production media and the strain with the highest activity was selected. The obtained strain examined on the fibrin plate and blood agar and was selected having the highest activity. The selected strain was identified by 16S rRNA analysis. The maximum fibrinolytic enzyme production of the selected strains was observed at 60 °C and pH 7,0. In the different culture conditions used for enzyme production, it has been found that the best carbon source is fructose and the best nitrogen sources are yeast extract and pepton. MgSO4, CaCl2 and KH2PO4 components were found to have a negative effect on the enzyme production and MnCl2, ZnC4H6O4 elements had a positive effect. The optimum nutrient composition to be used in enzyme production was determined as 0,5 g/L ZnC4H6O4, 0,5 g/L MnCl2, 14,22 g/L peptone, 11,190 g/L yeast extract, 14,22 g/L fructose.