Development and analytical applications of voltametric sensors for determination of florphenicol antibiotics

In this study, a sensitive voltammetric sensor was developed for the detection of florphenicol, a synthetic and long-acting antibacterial drug. Electrode modifications were made by cyclic voltammetry (CV) method using various monomers. Florphenicol voltammetric responses of 3- methyl thiophene (3-MTF) modified electrode were higher than those of other polymers electrodes. Then, The electrochemical behavior of florphenicol on 3-MTF modified glassy carbon electrode (GCE) was investigated by cyclic voltammetry and differential pulse voltammetry (DPV) method. In order to determine the florphenicol-sensitive sensor property of 3-MTF modified GCE; parameters such as electrolyte type and electrolyte pH, scan rate, film thickness, pulse amplitude, and pulse width have been optimized. When the florphenicol responses of the modified electrode were compared to the unmodified GCE responses, it was found that the selectivity, sensitivity and stability of the modified electrode and florphenicol responses were significantly increased. A linear calibration plot was obtained for florphenicol in the concentration range of 0.1 to 1.0 mM. The correlation coefficient from the linear calibration curve, R 2 , 0.999, determination limit, LOD, and quantitation limit, LOQ were calculated as 0.0399 mM and 0.1197 mM, respectively. In addition, analytical application of the voltammetric sensor developed for the determination of florphenicol was carried out in red and white meat samples. The recovery efficiency for red and white meat samples was obtained from 90.07% to 111.92% by using 3-MTF modified GC electrode. A method was developed for the florphenicol which is fast, simple, low-cost, 99.2% reproducible, stable, sensitive and selective.