The radiological and stereological analysis of the effect ofPRF, CGF and A-PRF on new bone formation, oral and maxillofacial surgery

The purpose of the study was to evaluate the influence of the PRF (Platelet Rich Fibrin), CGF (Concentrated Growth Factors) and A-PRF (Advanced Platelet Rich Fibrin) with or without autogenous bone(AB) on bone healing in surgically created critical-size defects (CSD) in the rat calvaria, by histological and stereological analysis. Eighty animals of 8 groups were used for surgical procedure, 15 rats used for cardiac puncture. A semilunar incision was made in the scalp on the anterior region of the calvarium. A 5-mm diameter CSD was created with a trephine bur. Defects of control group was left empty. Other groups were filled with autogenous bone, PRF, A-PRF, CGF, PRF with autogenous bone, A-PRF with autogenous bone and CGF with autogenous bone. The animals were sacrificed at 4 weeks. New bone formation was assessed by stereological and radiological analysis. The total percentage of newly formed bone was lowest in group empty and highest in group A-PRF with autogenous bone. Mean newly formed bone volumes between the A-PRF with autogenous bone group and autogenous bone group was statistically significant. Besides that, the difference between the A-PRF+AB group and PRF+AB group wasn't statistically significant in terms of the newly formed bone volume. Addition of A-PRF, PRF, and CGF have had significantly increased the bone formation at the 4th week. The effect of A-PRF, PRF, and CGF was similar and may be useful in the future to increase the success rate of bone grafting.