Preparing a new biosensor for glucose determination by immobilization of glucose oxidase in polyaniline-polyvinylsulphonate film

In this study, new amperometric biosensor for determination of glucose was developed. Polyaniline-polyvinylsulphonate-ferricyanide (PANI-PVS-FC) and polyaniline-polyvinylsulphonate-methylene blue (PANI-PVS-MB) films have been prepared on the platinum electrode by the electropolymerization of aniline was carried out in the presence of PVS, FC and MB. Optimum conditions of PANI-PVS-FC film was determined. Glucose oxidase enzyme has been immobilized in PANI-PVS-FC film via the entrapment method. Glucose detection is based on the oxidation of hydrogen peroxide and reduced form of ferricyanide at 0,3 V produced by the enzymatic reaction on the enzyme electrode surface. The linear working range of biosensor for glucose was determined. The effects of pH and temperature on the response of the glucose biosensor were investigated. Reusability and storage stability were determined of the biosensor. Interference effects were investigated on the amperometric response of the biosensor.