Evaluation of in vitro effects of glyphosate [N-(phosphonomethyl) glycine] (glyph) on quality and dna damageof rainbow trout (Oncorhynchus mykiss) spermatozoa

Today, the use of herbicides has increased and mixes with water sources in different ways. This situation does not affect the natural environment negatively. These substances can affect living things in the aquatic environment and cause them to accumulate in their bodies. This chemicals may also pose a danger to the consumer. Most studies conducted until today, have been on the adverse effects of pollution on the aquatic environment and aquatic organisms. For this purpose, in this study, it is aimed to investigate Glyphosate's (GlyPh) on possible in vitro exposure on the rainbow trout (Oncorhynchus mykiss) sperm, however, exotic for our country, that to be arbiter in aquaculture and produce 200.000 tons annually per year. Rainbow trout sperm were exposed to Glyphosate at various concentrations (2,5, 5, 10 mg L-1) in vitro at different concentrations. Sperm motility parameters were analyzed by computer assisted sperm analysis (SCA). DNA damage was analyzed to determine the extent to sperm cells are affected by toxic substances. With the increase of the applied concentration, sperm motility and duration decreased and DNA damage increased. Consequently, sperm cells are sensitive to low doses of Glyphosate and this can negatively affect natural populations.