Detection of virulence genes cause by motile Aeromonas spp. in Rainbow trout

In this thesis, we have studied bacterial fish disease as MotileAeromonas Septicemia caused by A. hydrophila, A. veronii, A. caviae and A.sobria in Rainbow trout at Mugla (Fethiye) region.Fish samples have been collected and carried out in cold and sterileconditions from rainbow trout farm as the fish showes symptones ofdiseases. The bacterial samples have been collected from different tisues andincubated in steril medium. DNA isolates was made from those samples todetect pathogen gene related to Motile Aeromonas Septicemia. We haveselected Aero gene and OmpTS gene from A. hydrophila, tet gene from A.veronii, F3-B3 gene from A. caviae and AH gene from A. sobria to detectbacteria. PCR was optimised and samples were electrophoresed.As a result of this study, A. hydrophila, A. veronii, A. caviae and A.sobria has seen as positive PCR results in liver and kidney tissue.