Isolation of Haloalkalopilic bacillus sp., production, characterization and determination of biotechnological application of their amylase, cellulase and xylanase

In this study, the amylase, cellulase, xylanase enzymes from Bacillus sp. strains isolated from Van lake soil were produced and characterized. The strains were tested for determination of temperature, pH range and NaCl concentration for growth and enzyme production on plate.Molecular weight of the amylase from Bacillus sp.AB-17 was estimated as 66.25 and 58 kDa on SDS-PAGE. The optimum activity was obtained at 150oC, pH 10.5, and the enzyme also presented halophilic properties with an activity around 67% between 0.5-3.4M NaCl concentration.Molecular weight of the Endoglucanase from Bacillus sp.C14 was detected as 61kDa on SDS-PAGE, and the optimum enzyme activity was obtained at 50oC, pH 11.0. Maximum endoglucanase activity (%133) was recorded at 20% (3.4M) NaCl.On the other hand the xylanase from Bacillus sp.X13 presented and optimum activity at 40oC and pH 6.0. The enzyme was 100% stable between pH 5.0-6.0 for 15 min. The enzyme was consist of 4 unit as 108.4, 95.5, 80.6 ve 68.5 kDa on SDS-PAGE.The results showed that the amylase AB-17 is a relevant enzyme for desizing processes in textile industries. The increased activity of C14 endoglucanase in high saline conditions, high halo-stability, resistance to detergents makes the enzyme C14 endoglucanase appropriate for textile, paper, animal feed preparation processes. X13 Xylanase enzyme could also be used for prebiotic preparation in food industries and fruit juice production without needing pectinase and cellulase usage.