In vitro propagation of Helianthemum germanicopolitanum Bornm

In this thesis, it was aimed to in vitro propagation and acclimatization of Helianthemum germanicopolitanum Bornm. plant which is a local endemic in Çankırı Province with arid and semi-arid lands, and an endangered (EN) species taking part among medicinal and aromatic plants. In this respect, 3 different basal media [a) Murashige and Skoog (MS) b) Gamborg's B5 c) Nitsch & Nitsch (N&N)], 2 different gelling agents (agar 7 g/L-gelrite 2.1 g/L), 8 cytokines and 8 auxin doses of plant growth regulators [a) 6-benzyladenin (BA)-(0 mg/L, 0,5 mg/L, 1 mg/L, 2 mg/L), b) Kinetin (KIN)-(0 mg/L, 0,5 mg/L, 1 mg/L, 2 mg/L), c) Indole-3-butyric acid (IBA)-(0 mg/L, 0.25 mg/L, 0.5 mg/L, 1 mg/L), d) α-napthaleneaceticacid (NAA)-(0 mg/L, 0.25 mg/L, 0.5 mg/L, 1 mg/L)] prepared in 64 different combinations with 30 g/L sucrose was added to the basal media and adjusted to pH 5.7 for in vitro propagation of H. germanicopolitanum. During the in vitro propagation of the plant, external and internal infections were frequently encountered and this was solved by the developed protocol. The best shoot growth (1.141) and shoot length (0.572) were obtained in the Gamborg's B5 basal media in combination with KIN (0.5 mg/L)+IBA (0.5 mg/L) PGR and solidified with gelrite. The maximum number of shoots (19.5) were obtained in the media containing BA (1 mg/L)+IBA (0.5 mg/L) PGR in MS media solidified with agar. At the rooting stage, the maximum number of roots (30) were reached in the MS media containing gelrite. In terms of shoot and root growth were taken to acclimatization stage 100 plants representing the best H. germanicopolitanum. 32 of these plants adapted to external conditions.