Relations of uremic toxins with apoptosis in hemodialysis patients

The objective of this study is to investigate the impact of both the uremic toxins (urea, creatinine, uric acid, calcium, phosphorus) and the hemodialysis treatment duration onto apoptosis process by measuring the level of M30 biomarkers which are generated by the caspase?s cleave of cytokeratins during this apoptosis process.Seventy volunteer patients that were composed of 34 female and 36 male patients who had undergone hemodialysis treatment with a mean age 55+14 and twenty healthy volunteers that were composed of 11 female and 9 male with a mean age 45 + 4 were included to this study. Hemodialysis patients were grouped on the basis of their treatment duration; 1st group was taken as a control group, 2nd group was selected between the ranges of 6 months-3 years; 3rd group was between the ranges of 4-8 years; 4th group was between the ranges 8-10 years of hemodialysis treatment.Whilst M30 levels were measured via ELISA methods, urea, creatinine, uric acid, phosphorus and calcium were obtained via spectrophotometric methods in blood samples which were taken prior to hemodialysis treatment.As a result, M30 levels of plasma (2nd group: 189 ± 71,7 U/L, 3rd group; 182 ± 65,2 U/L, 4th group; 288 ± 255,7 U/L ) were found significantly higher in hemodialysis patient groups when compared to the control group. (p<0,01) The correlations among M30 and uric acid in 2nd group (p<0,05, r: 0,449); M30 and urea in 3rd group (p<0,01, r: -0,521); M30 and uric acid in 4th group (p<0,01, r: 0,590) were found statistically significant. Additionally, the correlations were found statistically significant among M30 levels and uric acid (p<0,05, r: 0,291), phosphorus (p<0,05, r:0,265), calcium (p<0.05, r: -0,253) in whole hemodialysis patients.In conclusion, we suggest that apoptosis increases in hemodialysis patients and is closely correlated with serum phosphorus and uric acid levels but total duration of hemodialysis is not correlated with the apoptosis.