Preparing a new biosensor for hypoxanthine determination by immobilization of xanthine oxidase and uricasein polypyrrole-polyvinyl sulphonate film

In this study, new amperometric biosensor for determination of hypoxanthine was developed. For this reason; polypyrrole-polyvinylsulphonat films have been prepared on the platinum electrode by the electropolymerization of pyrrole was carried out in the presence of polyvinylsulphonate. Xanthine oxidase and uricase enzymes have been immobilized in polyanpyrrole-polyvinylsulphonate via the entrapment method. Optimum conditions of enzyme electrode was determined. Hypoxanthine detection is based on the oxidation of hydrogen peroxide at 0,4 V produced by the enzymatic reaction on the enzyme electrode surface. The linear working range of biosensor for hypoxanthine was determined. The effects of pH and temperature on the response of the hypoxanthine biosensor were investigated. Reusability and storage stability were determined of the biosensor. The performance of biosensor has been tested at real samples.